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Constipation is an extremely common symptom in the general population, (10-25% of US population)1. Rectum and anal sphincter play key roles in the defecatory process and continence function2. These disorders are also referred to as pelvic floor disorders. While there have been significant advances in our understanding of the normal and abnormal functioning of the anorectal function in health and disease, further progress is needed to advance diagnostic and treatment strategies of disorders related to anal sphincter malfunction. The goal of our study is to advance our understanding of the anal sphincter function in health and disease using novel diagnostic and treatment approaches.